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I-Blue Mini Plasmid Kit 300 Preps, Kits Includes: 65ml PD1 Buffer, 75ml PD2 Buffer, 100ml PD3 Buffer, 650uL I-Blue Lysis Buffer, 130ml W1 Buffer, 50ml Wash Buffer (Add Ethanol 200ml), 30ml Elution Buffer, 260ul RNase A (50MG per ML), 300 PD Columns, 300 2
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The I-Blue Mini Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7ml of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed in 20 minutes. The purified plasmid DNA is ready-for-use in restriction enzyme digestion, ligation PCR, and sequencing reactions.
The quality of the I-Blue Mini Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4ml overnight E. coli (DH5a) culture, containing plasmid pBluescript (A600>2U/ml). Following the purification process, a yield of more than 30µg is expected and the ratio of A260/A280 is between 1.8-2.0. The purified plasmid DNA (1µg) is used in EcoRI digestion and checked by electrophoresis.
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