Details

The Gel/PCR DNA Fragment Extraction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from an agarose gel, PCR, or any other enzymatic reaction. This method uses a chaotropic salt to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. The contaminants re washed with a wash buffer (containing Ethanol) and purified DNA fragments re eluted by a low salt based elution buffer or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.