Details

Introduction The miRNA Isolation Kit was designed for purifying micro RNAs (miRNAs) and other small cellular RNAs from tissue samples, cultured cells or fresh blood. Purification of miRNAs allows for research into biologically significant pathways for gene regulation. Standard protocols for isolating total RNA and mRNA are not optimized for isolation of small RNA molecules and result in the loss of substantial amounts of miRNAs and other small RNA. In addition, removal of the predominantly lager RNAs is required for accurate analysis of miRNA expression by qPCR or microarray analysis. This kit ensures purification of small RNA with minimal contamination from large RNA molecules or genomic DNA. In the presence of chaotropic salt, and various ethanol concentrations in the solvent, RNA molecules (of various sizes) are selectively bound by the glass fiber matrix (1).