Details

5X RNA Gel Loading Kit contains reagents for denaturing and loading RNA samples onto a formaldehyde gel, using MOPS as a buffer. The reagents are nuclease free and contain ultra pure deionized formamide and DEPC water. ?? The RNA sample is dissolved in 10µl of DEPC water and mixed with 35µl of denaturing solution. Heat the sample to 65??C for 5 minutes. Once the solution has cooled, add 5µl of loading dye. The sample is now ready to load into the gel.