Details

EndoFree Plasmid ezFlow Mini Kit 1 and 2: Plasmid isolated with traditional protocol normally contains high level
of endotoxins (lipopolysaccharides or LPS). For transfection of endotoxin sensitive cell lines or microinjection, the
endotoxins should be removed before the applications. The EZgene^TM endofree system uses a specially formulated
buffer that extracts the endotoxin from the plasmid DNA. Two rounds of extraction will reduce the endotoxin level
to 0.1 EU (Endotoxin) per µg of plasmid DNA. The endofree plasmid mini kit provides an efficient endotoxin removal
step into the traditional purification procedure to produce transfection grade plasmid DNA.

• Kit 1 Purification of plasmid 3-5 mL pf E.coli Culture.
• Kit 1 DNA Binding Capacity of up to 50  µg
• Kit 1 Yield around 8 to 12 µg
• Kit II Purification of plasmid 5-12mL of E. Coli
• Kit II DNA Binding Capacity of up to 30-70 µg