Details

• X-a-Gal
  [5-Bromo-4-Chloro-3-indolyl-a-D-galactopyranoside]

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  • CAS No. 107021-38-5
  • C₁₄H₁₅BrClNO₆
  • MW 408.6
  • Purity: >98%
  • Water Content: <0.05%

• Chromogenic substrate for detection of a-galactosidase activity in yeast Saccharomyces cerevisiae. Used in yeast Two-Hybrid System to detect protein-protein interactions within random libraries. The enzyme a-galactosidase enabels yeast to use disaccharide melibose as a carbon source during growth or fermentation. Secretion of this enzyme in response to activation of the GAL4 gene hydrolizes X-a-GAL and causes yeast colonies to develop a blue color. This reporter system allows immediate visible results on selection plates and eliminates necessity of a second screen filter life assay. Soluble in DMSO and DMF. Protect from light and moisture. Store at 2-8°C.