Product Description

Gel PCR DNA Fragment Extraction Kit, 4 Preps, Kit Contents: 4 DF Columns, 4 2ml Collection Tubes, 3ml DF Buffer, Wash Buffer (add Ethanol) 1ml (add Ethanol 4ml), Elution Buffer 1ml


    The Gel/PCR DNA Fragment Extraction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from an agarose gel, PCR, or any other enzymatic reaction. This method uses a chaotropic salt to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. The contaminants re washed with a wash buffer (containing Ethanol) and purified DNA fragments re eluted by a low salt based elution buffer or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.

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