Details

Details

QuickSilver™ TGS (Tris Glycine-SDS)

Tris Glycine-SDS is one of the most common running buffers used for SDS-PAGE. A 1X
solution contains 25mM Tris, 192mM Glycine and 0.1% SDS.

QuickSilver™ MES-SDS

 Small to medium molecular weight proteins can be resolved on SDS-PAGE gels using
MES-SDS as a running buffer. Proteins resolve faster in gels using MES-SDS as a running
buffer than those using MOPS-SDS. A 1X solution has a final concentration of 50mM Tris,
50mM MES, 0.1% SDS and 1mM EDTA.

QuickSilver™ MOPS-SDS

MOPS-SDS is ideal for use as a running buffer when resolving medium to large proteins
with SDS-PAGE. Gels run with MOPS-SDS buffer run slower than those using MES-SDS buffer.
The final concentration of a 1X working solution is 50mM Tris, 50mM MOPS, 0.1% SDS and
1mM EDTA.