Details

SmartCheck ladders are available in 3 size ranges
50bp, 100bp and 1kb to cover most common applications.  

• Protocol:  Briefly vortex the tube and use 5µl per lane. Additional loading buffer is not required.
  
  
• Concentration:  0.1 mg/ml
  
  
• Tracking dye: bromophenol blue and xylene cyanol
  
  
• Buffer formulation: 10mM Tris-HCl (pH 8.0), 5mM EDTA, 12.5% glycerol, 0.008% bromophenol blue, 0.008% xylene cyanol
  
  
• Shipping and Storage:  SmartCheck™ DNA Ladders are shipped at ambient temperature. On arrival, store at -20°C for optimum                                                                                              stability and long-term storage up to 15 months.
  
  
• Production:  SmartCheck™ ladders are produced from proprietary plasmids, digested to completion.  A multistep chromatography
                          method is used to ensure purity and DNA quality.
  
  
• Quality Control: Each lot of SmartCheck™ DNA Ladders is tested with a spectrophotometer to confirm DNA concentration, as
                                well as agarose electrophoresis to confirm band sharpness.