Details

rBAC Mini Total RNA Kits

The rBAC Mini Total RNA Kits have been specifically designed and optimized for Total RNA isolation from bacteria, including both gram-positive and gram-negative cultured bacterial cells. The gram-positive buffer, when combined with lysozyme, will efficiently lyse bacterial cell walls consisting of peptidoglycan. Detergents and chaotropic salt are then used to further lyse cells and inactivate RNase. Optional DNase treatments can be followed to remove unwanted DNA residue. RNA in the chaotropic salt is bound to the glass fiber matrix of the spin column. Contaminants are removed using a wash buffer and the purified RNA is eluted using RNase free water. The entire procedure can be completed in 30 minutes and the purified RNA is ready to use in RT-PCR, Northern Blotting, Primer Extension, mRNA Selection, and cDNA Synthesis. IBI Total RNA columns are individually packaged and certified RNase- and DNase-free.

The quality of the rBAC Mini RNA Bacteria Kit is tested on a lot-to-lot basis by isolating RNA from E. coli (1×10⁹) culture (OD600=1.3, 1ml) harvested by centrifugation at 16,000xg for 1 minute. 10µl from a 50µl eluate of purified RNA is analyzed by electrophoresis on a 0.8% agarose gel.