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Tryptose Phosphate Broth is a medium recommended for the cultivation of fastidious microorganisms. Trypose phosphate broth is a buffered broth which can support the growth fastidious streptococci and meningococci. The addition of 0.1 agar concentration can aid in the recovery of obligate anaerobes. Tryptose peptone and dextrose are a source of carbon, organic nitrogen, and energy. Sodium chloride contributes to osmonic equilibrium, and disodium phosphate is a buffering agent