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Biomiga One-Step RT-PCR Kit, 25 Preps, total RNA can range from 0.01pg to 2ug, Kit Includes MMLV RT Hot start Taq Mix, 2X Reaction mix, 5mm Magnesium Sulfate.
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One-Step RT-PCR: Is designed for the reverse transcription (RT) and polymerase chain reaction(PCR) amplification of a specific target RNA from either total RNA or mRNA. This system uses amixture of MMLV Reverse Transcriptase and Hot start Taq DNA polymerase in an optimized reactionbuffer, and can detect RNA targets up to 4.0 kb. The amount of input total RNA can range from 0.01pg to 2 μg. Sufficient reagents are provided for 25 or 100 amplification reactions of 50 μl each.
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