Details

Mollusc gDNA Isolation Kits: is designed to extract genomic DNA up to 60 kb in size from
molluscs, insects, arthropods, roundworms, flatworms, and other invertebrate tissue samples
rich in mucopolysaccharides. The method is suitable for invertebrates frozen or preserved in
alcohol or DNE solution, and good results can be obtained with formalin preserved material.
Samples are homogenized and lysed in a high salt buffer and extracted with chloroform to
remove mucopolysaccharides. Following a rapid alcohol precipitation step, binding conditions
 are adjusted and DNA further purified using ezBind DNA spin columns. While proteins and other
 contaminants are removed by wash buffer, high quality genomic DNA is eluted with elution buffer
 or sterile water. The purified genomic DNA is suitable for downstream applications such as Southern
 Blot, restriction digestion, and PCR.