Details

• X-GlcA Cyclohexylammonium Salt
  [5-Bromo-4-chloro-3-indolyl-ß-D-glucuronic acid, cyclohexyl ammonium salt]

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  • CAS No. 18656-96-7
  • C₂₀H₂₆BrClN₂O₇
  • MW 521.8
  • Purity: >99%

• Histochemical substrate for B-D-Glucuronidase (GUS) encoded by the gusA gene. The substrate is used as a qualitative histochemical marker of specific GUS expression in cells and tissue. X-GlcA is cleaved by GUS at the B1 glucuronic bond between glucuronic acid and the 5-Bromo-4-chloro-3-indolyl part of X-GlcA via hydrolysis. The cleavage of X-GlcA results in the precipitation of water insoluble blue dichloro-dibromo-indigo precipitate at the site of enzymatic cleavage. Soluble in DMSO and DMF. Allow to warm to room temperature before using. Store at 0-5°C.